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KMID : 0545119950050040238
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 4 p.238 ~ p.240
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A Simple Method for Recovery of Microbial Poly-β-hydroxybutyrate byAlkaline Solution Treatment
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Lee, In Young
Chang, Ho Nam/Park, Young Hoon
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Abstract
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A novel and simple purification method for microbial poly-β-hydroxybutyrate (PHB) was developed. Sodium hydroxide was found to be efficient for digesting cell materials. Initial biomass concentration, NaOH concentation, digestion time, and incubation temperature were optimized. When 40 g/ℓ of biomass was incubated in 0.1 N NaOH at 30℃ for 1 h, PHB purity of 88.4% with a weight average molecular weight (M_w) of 770,000 and a polydispersity index (PI) of 2.4 was recovered with a yield of 90.8 % from the biomass which initially contained PHB of a M_w of 780,000 and a PI of 2.3.
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